Sigma

3X FLAG® Peptide

F4799 -

lyophilized powder

DOWNLOAD MSDS (PDF)

Synonym: Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys

  • Empirical Formula (Hill Notation): C120H169N31O49S

  • Molecular Weight: 2861.87

  • MDL number: MFCD01863911

Properties

Related CategoriesFLAG Affinity Purification, FLAG Peptides, FLAG System, Plant Proteomics, Protein Purification,
formlyophilized powder
concentration (Recommended working concentration is 100 μg/ml for elute 3X FLAG fusion proteins from the ANTI-FLAG® M2 affinity gel.)
shipped inwet ice
storage temp.−20°C
 2-8°C

Description

Other Notes

To view FLAG® technology literature, visit www.sigma-aldrich.com/flagliterature.

Preparation Note

To prepare a stock solution, dissolve in TBS (50 mMTris-HCl, pH 7.4, with 150 mM NaCl) at a concentration of 5 mg/ml.

Application

For use in competitive elution of 3X FLAG® fusion proteins from the ANTI-FLAG® M2 monoclonal antibody in solution or bound to agarose on the ANTI-FLAG® M2 agarose affinity gel. This is achieved by Affinity Chromatography.

General description

The 3X FLAG Peptide is a synthetic peptide of 23 amino acid residue. The Asp-Tyr-Lys-Xaa-Xaa-Asp motif is repeated three times in the peptide. Eight amino acids at the C-terminus make up the classic FLAG sequence (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys).

Legal Information

ANTI-FLAG is a registered trademark of Sigma-Aldrich Co. LLC

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

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Documents

Certificate of Analysis

Certificate of Origin

F4799 - Datasheet (78KB)
Proteomics Prod Guide (141 KB)
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Safety Information

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany3
Technical information & documentation associated with this product is available in the Safety & Documentation tab.

References

Kumagai,A et al., TopBP1 Activates the ATR-ATRIP Complex Cell 124(5), 943-955, (2006)

Doyon,Y et al., Structural and Functional Conservation of the NuA4 Histone Acetyltransferase Complex from Yeast to Humans Mol. Cell. Biol. 24(25), 1884-1896, (2004)

Warren,E et al., Development of a Protein Chip: A MS-Based Method for Quantitation of Protein Expression and Modification Levels Using an Immunoaffinity Approach Anal. Chem. 76(14), 4082-4092, (2004)


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